Open AccessMeasurement of Leptin by RIA Versus MIA in a Population of Healthy Newborns
Author(s) -
TreviñoGarza Consuelo,
MancillasAdame Leonardo,
EstradaZúñiga Cynthia M.,
VillarrealPérez Jesús Z.,
VillarrealMartinez Laura,
De la OCavazos Manuel E.
Publication year - 2016
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21845
Subject(s) - concordance , medicine , bland–altman plot , radioimmunoassay , concordance correlation coefficient , leptin , immunoassay , population , correlation coefficient , umbilical cord , coefficient of variation , limits of agreement , statistics , immunology , antibody , mathematics , nuclear medicine , environmental health , obesity
Background Assays based on multiplex immunoassay (MIA) technology have demonstrated advantages over enzyme‐linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Its acceptance depends on how well it performs in comparison to older techniques. The aim is to compare the results of leptin using RIA versus MIA. Methods We analyzed 81 samples of umbilical cord blood of healthy term newborns by RIA and MIA. Results The concordance correlation coefficient was 0.158 (95% CI 0.10–0.21). Pearson's correlation coefficient was 0.6651 (95% CI 0.52–0.77; P < 0.0001). In the Bland–Altman plot, concordance is acceptable because most of the measurements are within a mean of ±1.96 SD. Conclusions As shown by the Bland–Altman plot, there is concordance by both methods, but with a weak correlation.