Open AccessComparison of Chromogenic In Situ Hybridization and Fluorescence In Situ Hybridization for the Evaluation of MDM2 Amplification in Adipocytic Tumors
Author(s) -
Mardekian Stacey K.,
Solomides Charalambos C.,
Gong Jerald Z.,
Peiper Stephen C.,
Wang ZiXuan,
Bajaj Renu
Publication year - 2015
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21794
Subject(s) - cish , chromogenic in situ hybridization , fluorescence in situ hybridization , biology , in situ hybridization , gene duplication , chromogenic , liposarcoma , fish <actinopterygii> , in situ , microbiology and biotechnology , pathology , sarcoma , medicine , genetics , chemistry , gene , chromosome , gene expression , organic chemistry , chromatography , fishery
Background Atypical lipomatous tumor/well‐differentiated liposarcoma (ALT‐WDLPS) and dedifferentiated liposarcoma (DDLPS) are characterized cytogenetically by a 12q13–15 amplification involving the mouse double minute 2 ( MDM2 ) oncogene. Fluorescence in situ hybridization (FISH) is used frequently to detect this amplification and aid with the diagnosis of these entities, which is difficult by morphology alone. Recently, bright‐field in situ hybridization techniques such as chromogenic in situ hybridization (CISH) have been introduced for the determination of MDM2 amplification status. Methods The present study compared the results of FISH and CISH for detecting MDM2 amplification in 41 cases of adipocytic tumors. Amplification was defined in both techniques as a MDM2 /CEN12 ratio of 2 or greater. Results Eleven cases showed amplification with both FISH and CISH, and 26 cases showed no amplification with both methods. Two cases had discordant results between CISH and FISH, and two cases were not interpretable by CISH. Conclusion CISH is advantageous for allowing pathologists to evaluate the histologic and molecular alterations occurring simultaneously in a specimen. Moreover, CISH is found to be more cost‐ and time‐efficient when used with automation, and the signals do not quench over time. CISH technique is a reliable alternative to FISH in the evaluation of adipocytic tumors for MDM2 amplification.