Open AccessAssociation Between Genetic Polymorphism of the MIF Gene and Colorectal Cancer in Taiwan
Author(s) -
Ramireddy Latha,
Chen William TzuLiang,
Peng ChingTien,
Hu RouhMei,
Ke TaoWei,
Chiang HuaChe,
Chang ShengChi,
Tsai FuuJen,
Lo WanYu
Publication year - 2015
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21763
Subject(s) - single nucleotide polymorphism , snp , genotyping , colorectal cancer , genotype , macrophage migration inhibitory factor , snp genotyping , medicine , oncology , gastroenterology , biology , cancer , gene , genetics , cytokine
Background Colorectal cancer (CRC) is the highest leading cause of cancer‐related mortality in Taiwan. Macrophage migration inhibitory factor (MIF) has recently been defined as a novel protumorigenic factor that promotes cell proliferation, migration, and invasion. The aim of the present study is to identify the association between MIF gene polymorphism and CRC. Methods A case–control study was designed to test the hypothesis. A total of 192 biopsy‐diagnosed CRC patients (CRC) and 256 healthy subjects (control) were recruited. Genotyping of four single nucleotide polymorphism (SNPs; rs755662, rs11548059, rs1049829, rs1803976) at chromosome positions 755662 (5′ UTR), 11548059 (exon2), 1049829 (exon2), 1803976 (exon3) was performed using a Taqman SNP genotyping assay. Results There is a significant difference in genotype frequency distribution of rs755662 polymorphism between CRC patients and controls ( P = 0.011). No significant difference was found in the frequency distribution of rs11548059, rs1049829, rs1803976 polymorphism in CRC patients and controls ( P = 0.660, P = 0.700, and P = 0.959, respectively). Moreover, the MIF‐173 SNP was also significantly associated with young patients (age < 50 years, P = 0.026) late stage (Stage IV, P = 0.038) and poor differentiation group ( P = 0.040). Compared to the control group, the MIF‐173 SNP also significantly associated with patients with stages III and IV ( P = 0.034 and 0.003, respectively). Conclusion The presence of MIF ‐173 (G/C) gene polymorphism (rs755662) was associated with susceptibility, patient age, and stages of CRC in Taiwanese.