Open AccessSoluble l ‐Selectin as an Independent Biomarker of Bronchial Asthma
Author(s) -
Nadi Ebrahim,
Hajilooi Mehrdad,
Pajouhan Saeed,
Haidari Mehran
Publication year - 2015
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21749
Subject(s) - odds ratio , asthma , confidence interval , medicine , biomarker , gastroenterology , receiver operating characteristic , body mass index , immunology , endocrinology , chemistry , biochemistry
Background We sought to determine the association of plasma level of soluble l ‐selectin (sL‐selectin) and F206L polymorphism of l ‐selectin with asthma. Methods A total of 90 asthmatic patients and 90 sex‐ and age‐matched healthy controls were enrolled. The plasma level of s l ‐selectin was measured by enzyme‐linked immunosorbent assay (ELISA) method. An amplification refractory mutation system polymerase chain reaction was performed to detect F206L polymorphism of l ‐selectin. Results The mean plasma levels of s l ‐selectin was significantly higher in the patients with asthma than the controls (2113 ± 466 vs. 1664 ± 322 ng/ml, P = 0.001). Logistic regression analysis after adjustment for age, sex, and body mass index demonstrated that plasma levels of s l ‐selectin are an independent biomarkers for asthma (odds ratio [OR], 1.86; 95% confidence interval [95% CI], 1.42–2.24). The area under the receiver operating characteristic (ROC) curve for s l ‐selectin was 0.792, 95% CI (0.732–0.862), P = 0.0001. Individuals with the minor homozygote of F206L polymorphism of l ‐selectin demonstrated a higher level of s l ‐selectin than the major homozygous (2319 ± 732 vs. 1917 ± 453 ng/ml, P = 0.02). No association was found between F206L polymorphism of l ‐selectin with asthma. Conclusion Our study suggests that plasma level of s l ‐selectin is an independent biomarker for asthma.