Open AccessTechnology and New Fluorescence Flow Cytometry Parameters in Hematological Analyzers
Author(s) -
Arneth Borros M.,
Menschikowki Mario
Publication year - 2015
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21747
Subject(s) - hematology analyzer , cell counting , spectrum analyzer , blood cell , flow cytometry , blood smear , pathology , cytometry , biomedical engineering , medicine , cell , computer science , immunology , biology , telecommunications , genetics , malaria , cell cycle
Background Modern hematological analyzers offer a series of new tests in addition to the differential blood cell count. Here, we provide a short overview of the technique and the new tests included in the hematological analyzers manufactured by the company Sysmex (Kobe, Japan). As introduced here, the resulting new extended blood cell count allows for the quantification of DNA‐ and RNA‐rich, activated and/or precursor leukocyte subpopulations. Methods To compare the different methods of blood cell counting, repetitive cell counting is carried out by hematological experts using microscopy and using the XE‐5000 hematological analyzer. Results The hematological analyzer delivers more‐accurate results with respect to normal cells but microscopy is suited better to recognize and count pathological cells. Conclusion With the new hematological analyzers, several new hematological tests are made available as screening tools that were not possible previously using standard cell‐counting procedures.