Open AccessClinical Usefulness of the PAXgene TM Bone Marrow RNA System for Stabilizing Total RNA
Author(s) -
Takeda Mayu,
Funato Tadao,
Ikemoto Masaki,
Nanmoku Toru,
Urasaki Yoshimasa,
Iwatani Yoshinori
Publication year - 2015
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21729
Subject(s) - rna , rna extraction , bone marrow , reverse transcriptase , peripheral blood , microbiology and biotechnology , gene , biology , biochemistry , immunology
The collection of clinical samples, such as bone marrow (BM) and peripheral blood, is an important procedure for the extraction of the cellular RNA. It is essential to preserve the extracted RNA during and after the collection of clinical samples to ensure the accurate analysis of gene expression. To date, the PAXgene TM Blood RNA System has been proven useful for stabilizing RNA extracted from peripheral blood; however, a problem concerning the stability of the total RNA stored using the system has been identified. The PAXgene TM Bone Marrow RNA System (BM system) is a newly developed system, and its clinical usefulness as a stabilizer for the cellular RNA in BM and peripheral blood was investigated with respect to the quality of RNA extracted using this system. A quantitative reverse transcriptase polymerase chain reaction (RT‐PCR) was carried out using total RNA extracted with the BM system, which showed that total RNA was more stable in the BM system than in the conventional system, indicating that the BM system can be applied to RT‐PCR. The BM system enabled us to detect Wilms’ tumor suppressor gene (WT1) more effectively than the conventional system. In conclusion, the BM system is clinically valuable for extracting and stabilizing total RNA of high quality.