Open AccessEvaluation of the HerpeSelect Express Rapid Test in the Detection of Herpes Simplex Virus Type 2 Antibodies in Patients With Genital Ulcer Disease
Author(s) -
AlShobaili Hani,
Hassanein Khaled M.,
Mostafa Marwa Salah,
Duways Ali Saleh Al
Publication year - 2015
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21725
Subject(s) - herpes simplex virus , genital ulcer , virology , immunoassay , antibody , monoclonal antibody , gold standard (test) , medicine , alphaherpesvirinae , immunology , virus , viral disease , herpesviridae , sexually transmitted disease , human immunodeficiency virus (hiv) , syphilis
Background A rapid point‐of‐care test with high sensitivity and specificity is required in order to fulfill the need for early detection and screening of Herpes simplex virus type 2 (HSV‐2) infection among patients with genital ulcer disease (GUD), for better management and control of virus transmission. Methods The goal of this study is to evaluate the performance of the commercially available HerpeSelect Express rapid test in comparison with three ELISA assays: HerpeSelect ELISA, Kalon HSV‐2 glycoprotein G2 assay, and monoclonal antibody blocking enzyme immunoassay, which was used as the gold standard for the detection of HSV‐2 antibodies. Results This study showed high sensitivity (ranging from 82.6 to 100%) and specificity (100%) of the HerpeSelect Express rapid test when compared to the three ELISA assays. The agreement between the HerpeSelect Express rapid test with the three ELISAs ranged from 93.3 to 100%. Conclusion The HerpeSelect Express rapid test has adequate sensitivity and specificity for confirming HSV‐2 infection in patients with GUD, indicating its suitability for epidemiological studies.