Open AccessInability of Monoclonal Anti‐light Chain Antibody to Detect Clonal B‐Cells in a Patient with Follicular Lymphoma by Multicolor Flow Cytometry
Author(s) -
Velzen Jeroen F.,
den Blink Dorine,
Wiegers Ingrid E.H.,
Bloem Andries C.
Publication year - 2014
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21716
Subject(s) - monoclonal antibody , flow cytometry , immunoglobulin light chain , follicular lymphoma , monoclonal , antibody , microbiology and biotechnology , lymphoma , biology , immunology
Background Our recent publication “Inability of a monoclonal anti‐light chain antibody to detect clonal plasma cells in a patient with multiple myeloma by multicolor flow cytometry,” underlined the importance of choice of antibodies to detect cytoplasmic light chains. Our present study extends this finding for detection of surface immunoglobulin ( SI g) light chains on clonal B ‐cells. Methods Multicolor flow cytometry was used for analyzing bone marrow ( BM ) from a patient with a CD 10‐positive follicular lymphoma for infiltrating clonal B ‐cells. Results In the BM aspirate, B cells could be identified expressing CD 19, CD 10, and high levels of CD 20. No SI g light chain expression was found on this population of B cells employing monoclonal antibodies. Re‐analysis using polyclonal antibodies against SI g light chains, revealed presence of lambda light chains on the CD 10positive B ‐cells. Conclusions These data illustrate when antibodies against SI g light chains are employed for B ‐cell clonality assessment, polyclonal antibodies are preferred over monoclonal antibodies.