Open AccessRapid and Sensitive Headspace Gas Chromatography–Mass Spectrometry Method for the Analysis of Ethanol in the Whole Blood
Author(s) -
Xiao Hongtao,
He Lin,
Tong Rongsheng,
Yu Jiying,
Chen Lu,
Zou Jing,
Li Jinqi,
Bian Yuan,
Zhang Yuan
Publication year - 2014
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21698
Subject(s) - chromatography , detection limit , chemistry , mass spectrometry , selected ion monitoring , gas chromatography–mass spectrometry , gas chromatography , quantitative analysis (chemistry) , isothermal process , analytical chemistry (journal) , linear range , physics , thermodynamics
Background For the forensic aim, a sensitive and specific method using headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in blood using n ‐propanol as internal standard. GC was performed in isothermal mode with a GC run‐time of 5.0 min. Methods The quantification was performed using selected ions monitoring mode adopting a quantitative ion and qualifier ion for ethanol and the internal standard. Results The method was linear ( r 2 = 0.999, in the concentration range of 39.5–1,262.9 μg/ml), specific, sensitive (limit of quantification and limit of detection of 39.5 and 0.4 μg/ml, respectively), and robust. A slightly modified method was also developed for the quantification of 50 commonly abused drunken in blood. The method used an isothermal GC program with a run‐time of 5.0 min. The quantification was performed using selected ions monitoring mode and integrating the area under the peak using n ‐propanol as an internal standard. The method was linear 40–1,263 μg/ml and sensitive. Conclusions The method was proved superior in speed and selectivity to previously reported methods and was successfully applied to the pharmacokinetic study of ethanol.