Open AccessReappraisal of Quantitative Gel Zymography for Matrix Metalloproteinases
Author(s) -
Prescimone Tommaso,
Tognotti Danika,
Caselli Chiara,
Cabiati Manuela,
D'Amico Andrea,
Del Ry Silvia,
Giannessi Daniela
Publication year - 2014
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21696
Subject(s) - zymography , matrix metalloproteinase , gelatinase , quantitative analysis (chemistry) , human plasma , chromatography , chemistry , extracellular matrix , matrix (chemical analysis) , biochemistry
Background The determination of matrix metalloproteases (MMPs) is relevant in many pathophysiological conditions, especially if associated with extracellular matrix remodeling; however, the results obtained are closely linked to the method used and are not directly comparable. The aim of this study was to perform a reappraisal of quantitative gel zymography technique for MMPs in human plasma, to use for comparison with commercially available ELISA and in those experimental conditions where the MMP active form needs to be revealed. Methods The critical methodological parameters of zymography were checked and a comparison with a routinely used ELISA was performed. Results Sensitivity and reproducibility levels of zymography are suitable for detection of MMP‐9 in human plasma, providing results closely related to those obtained by ELISA. Conclusions Analytical parameters of zymography were suitable for detection of MMPs in human plasma. Quantitative zymography for MMPs is an alternative method for comparing the results of ELISA widely employed for MMP determination, thus reducing the discrepancies between laboratories regarding gelatinase assay.