Open AccessStability of [−2]Pro‐PSA in Whole Blood and Serum: Analysis for Optimal Measurement Conditions
Author(s) -
Igawa Tsukasa,
Takehara Kosuke,
Onita Toru,
Ito Kazuto,
Sakai Hideki
Publication year - 2014
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21687
Subject(s) - prostate specific antigen , prostate cancer , medicine , whole blood , urology , prostate , cancer
Background The clinical usefulness of [−2]pro‐PSA (where PSA is prostate‐specific antigen) in prostate cancer diagnosis has been emphasized in recent studies. To determine proper blood sample handling conditions for [−2]pro‐PSA evaluation, we analyzed the preanalytical stability of [−2]pro‐PSA. Methods Blood samples from 22 Japanese males were stored under various conditions before total PSA (tPSA), free PSA, and [−2]pro‐PSA concentrations were measured, and the preanalytical stability of [−2]pro‐PSA and the changes in the Prostate Health Index ( phi ) were assessed. Results [−2]Pro‐PSA was stable in serum for at least 24 hr at both room temperature (RT) and at 4°C. However, [−2]pro‐PSA levels in whole blood increased rapidly over time, particularly at RT. Mean recovery (%) of [−2]pro‐PSA in whole blood at RT was >110% at 1 hr after drawing of blood. The phi tended to increase over time in a pattern similar to the change in[−2]pro‐PSA. Conclusions Preanalytical stability was lower for [−2]pro‐PSA than for free PSA or tPSA. Whole‐blood [−2]pro‐PSA increased in a time‐dependent manner, particularly at RT. Thus, whole blood samples collected at RT should be centrifuged within 1 hr after drawing. The [−2]pro‐PSA in serum is stable for at least 24 hr at both RT and at 4°C.