Open AccessSimplified Microchip Electrophoresis for Rapid Separation of Urine Proteins
Author(s) -
Song Hongwei,
Wang Huimin,
Ju Saoqing,
Jin Qinghui,
Jia Chunping,
Cong Hui
Publication year - 2014
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21651
Subject(s) - proteinuria , chromatography , chemistry , urine , agarose gel electrophoresis , detection limit , albumin , capillary electrophoresis , electrophoresis , transferrin , biochemistry , medicine , kidney , gene
Background Urine protein test has been widely used in clinics, but to determine the type of proteinuria is usually difficult due to technical limitations. Methods In the current study, a rapid and simple method to separate and determine urine proteins by a microchip electrophoresis (ME) system has been developed in which only 4 min are required. Results Optimal separation conditions have been established by using 15 s injection time at 500 and 1,500 V separation voltage in 75 mmol/l borate buffer containing 0.8 mmol/l calcium lactate and 1% ϕ ethylamine (pH 10.55). Relative standard deviation (RSD) of migration time with purified human albumin and human transferring was 2.68% and 2.24%, and RSD of the peak area was 5.85% and 4.96%, respectively. The linear detection range was 1.0–15.0 g/l for purified human albumin and 1.0–10.0 g/l for human transferrin, with the same detection limit (S/N = 3) of 0.4 g/l. Finally, comparing to conventional agarose gel electrophoresis, the same results were obtained by using ME by testing clinical samples including 60 selective proteinuria, 105 nonselective proteinuria, and 6 overflow proteinuria. Conclusion This newly established ME could have broad applications to determine the type of proteinuria in clinics.