Open AccessOxidative Stress‐Related Enzyme Polymorphisms Associated With the Immunological Biomarkers Levels in Heavy Drinkers in Taiwan
Author(s) -
Tseng YangMing,
Tsai ShihMeng,
Lin ChunChin,
Jin YiRu,
Yeh WeiHao,
Hsiao JenKuei,
Chen ChiFen,
Lan WuHsiang,
Tsai LiYu
Publication year - 2013
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21633
Subject(s) - oxidative stress , superoxide dismutase , myeloperoxidase , malondialdehyde , genotype , catalase , biology , immunology , antibody , glutathione peroxidase , biochemistry , gene , inflammation
Background Excessive alcohol intake can result in the oxidative stress in cells and the genetic variations of alcohol‐metabolizing enzymes are responsible for the different degrees of toxicity of alcohol in several organs, such as the liver and immunological systems. We hypothesized that the alteration of oxidative stress due to some genetic variations of oxidative stress‐related enzymes could result in changes of specific biomarkers, and heavy drinkers could be cautioned about the predictive likelihood to induce drinking‐induced diseases. Methods A total of 108 heavy drinkers and 106 nonheavy drinkers were enrolled and the hematological, biochemical, and immunological tests were measured; the genotypes of oxidative stress‐related enzymes, including manganese superoxide dismutase ( MnSOD 1183T>C), glutathione peroxidase 1 ( GPX1 Pro198Leu), catalase ( CAT ‐262C>T), and myeloperoxidase ( MPO ‐463G>A), were assayed by real‐time polymerase chain reaction (PCR) and PCR‐restriction fragment length polymorphism (PCR‐RFLP). Results For the males, the levels of carbohydrate‐deficient transferrin (CDT), malondialdehyde (MDA), CD4 + , immunoglobulin G (IgG), immunoglobulin M (IgM), and IL‐6 were significantly different between the two groups. Furthermore, there were higher proportions of CD19 + cells and lower TNF‐α levels in heavy drinkers with the MnSOD C carriers, and there were higher percentages of CD19 + cells and IL‐6 levels in heavy drinkers with the combined genotypes of MnSOD C carriers and MPO A carriers. Conclusions Our findings indicate that heavy drinkers may be cautioned predictive likelihood for them to induce drinking‐induced diseases by analyzing their MnSOD genotypes and immunological biomarkers.