Open AccessMeasurement of Serum Total Vitamin D (25‐OH) Using Automated Immunoassay in Comparision With Liquid Chromatography Tandem‐Mass Spectrometry
Author(s) -
Shin SangYong,
Kwon MinJung,
Song Junghan,
Park Hyosoon,
Woo HeeYeon
Publication year - 2013
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21598
Subject(s) - vitamin d deficiency , immunoassay , liquid chromatography–mass spectrometry , vitamin d and neurology , tandem mass spectrometry , medicine , vitamin , chromatography , population , chemistry , mass spectrometry , endocrinology , immunology , antibody , environmental health
Background The associations of vitamin D deficiency with many nonskeletal diseases are still being discovered. We evaluated the use of an automated immunoassay to measure serum total vitamin D (25‐OH) and assessed vitamin D status in a Korean adult population. Methods We compared the Elecsys Vitamin D (25‐OH) Total Assay (Roche Diagnostics) with liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) using 300 serum samples. Total imprecision was calculated using three levels of quality control materials and serum samples. We also investigated the vitamin D status using data for 70,762 cases who had a routine health check‐up in our hospitals. Results The regression equation: Elecsys = 0.882 × LC‐MS/MS + 6.814 ( r = 0.926). Total imprecision was within 10% for all quality control materials and serum samples. The prevalence of vitamin D deficiency using cut‐off values of <50 nmol/l (<20 ng/ml) were 70.3% in males and 86.4% in females, respectively. The prevalence of vitamin D deficiency was higher in younger subjects than in older subjects ( P for linear‐by‐linear association was <0.001). Serum vitamin D levels were highest in September and lowest in February. Conclusion The Elecsys Vitamin D (25‐OH) Total Assay was comparable to LC‐MS/MS and appropriate for routine clinical use. Vitamin D deficiency is common in Korean adults.