Open AccessImportance of Collection Methods and Stability of Oral Fluid Samples for Hepatitis B Surface Antigen Detection
Author(s) -
de Paula Scalioni Letícia,
Cruz Helena Medina,
Paula Vanessa Salete,
Corrêia Oliveira Jaqueline,
Tourinho dos Santos Renata,
MottaCastro Ana Rita Coimbra,
Murat Paula Guerra,
VillelaNogueira Cristiane Alves,
LewisXimenez Lia Laura,
Lampe Elisabeth,
Villar Livia Melo
Publication year - 2013
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21582
Subject(s) - hbsag , receiver operating characteristic , chromatography , detection limit , hepatitis b , sample (material) , hepatitis b virus , medicine , virology , chemistry , virus
Background Oral fluid ( OF ) sample collection and stability for HB s A g detection are not fully established. This study aims to investigate the applicability of OF collectors and sample stability for Hepatitis B virus surface antigen detection. Methods Paired serum and OF samples were obtained from 191 individuals, and Chembio (Chembio Diagnostic System, USA) and Salivette (Sarstedt, Germany) devices were used for OF collection. Two HB s A g enzyme immunoassays ( EIA s) were used ( HB s A g One kit, Radim, Rome, Italy and ETI‐MAK‐4, DiaSorin, Vercelli, Italy) to determine the most efficient method according OF collector. Sample volume, incubation time, and cutoff ( CO ) value were evaluated. The stability of OF samples was determined under different environmental conditions. Results Chembio samples analyzed using DiaSorin EIA without modification of the manufacturer's instructions, demonstrated a sensitivity of 95.24% and a specificity of 100%. Salivette samples analyzed with Radim EIA with receiver operating characteristic ( ROC ) curve for calculating the CO showed a sensitivity of 78.26% and a specificity of 89.88%. HB s A g was detected in Chembio and Salivette samples under different environmental conditions, but the Chembio samples were the most stable. Conclusions Both collectors can be used for HB s A g detection in OF samples, but some modifications of commercial EIA s should be incorporated for Salivette device. OF samples were reliably stable and could be stored for up to 90 days at 2–8°C.