Open AccessPredominance of E nterobacteriaceae Isolates in Early Positive Anaerobic Blood Culture Bottles in B ac T /Alert System
Author(s) -
Chiueh TzongShi,
Lee ShihYi,
Tang ShengHui,
Lu JangJih,
Sun JunRen
Publication year - 2013
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21571
Subject(s) - anaerobic exercise , microbiology and biotechnology , anaerobic bacteria , blood culture , bacteria , microorganism , biology , aerobic bacteria , food science , physiology , antibiotics , genetics
We collected and analyzed the time to detection ( TTD ) of blood cultures in the B ac T / A lert automated system from 2002 to 2007. Among the 10,893 monomicrobial isolates from a total of 133,735 blood culture sets, the recoveries of aerobic bottles were compared with those of anaerobic bottles in this study. Significantly more G ram‐positive cocci (except S taphylococcus aureus and enterococci), glucose nonfermentative G ram‐negative bacteria, and yeast were recovered from aerobic bottles than from anaerobic bottles. The average TTD was 19.0 hr and 20.1 hr for the aerobic and anaerobic bottles, respectively, and 96.8% of the microorganisms were detected within the first 72 hr. Of the 5,489 microorganisms recovered from both of the blood culture bottle pair, microbial growth was significantly more often detected first in the anaerobic bottles than the aerobic bottles for E nterobacteriaceae except S erratia marcescens , while S . aureus , coagulase‐negative staphylococci and P seudomonas aeruginosa were more often detected first in the aerobic bottles. According to these data, we conclude that the earlier positivity of anaerobic bottles is a useful marker for rapid presumptive identification of E nterobacteriaceae infection. J. Clin. Lab. Anal. 27:113–120, 2013. © 2013 Wiley Periodicals, Inc.