Open AccessFlow Cytometry Immunophenotyping Evaluation in Acute Lymphoblastic Leukemia: Correlation to Factors Affecting Clinic Outcome
Author(s) -
Vasconcelos de Andrade Alves Gabriela,
Araújo da Cunha Fernandes Andrea Luciana,
Freire Juliana Mendonça,
de Souza Paiva Aldair,
de Vasconcelos Roberto Chaves,
Soraya de Farias Sales Valéria,
Maria de Araújo Moura Lemos Telma,
Gil Erica Aires,
Miranda de Araújo Freire Flávio Henrique,
Silva Dany Geraldo Kramer Cavalcanti e,
Maciel James Farley Rafael,
Farkatt Irian Guedes,
Júnior Geraldo Barroso Cavalcanti
Publication year - 2012
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21540
Subject(s) - immunophenotyping , flow cytometry , t cell , medicine , pathology , immunology , b cell , leukemia , lymphoma , antibody , immune system
The authors conducted a flow cytometry immunophenotyping study in patients with acute lymphoblastic leukemia ( ALL ) from N atal, R io G rande do N orte, B razil. The patients ( n = 126) were newly diagnosed using a panel of monoclonal antibodies: CD 1a, CD 2, CD 3, CD 4, CD 7, CD 8, CD 10, CD 13, CD 33, CD 14, CD 19, CD 22, CD 79a, CD 117, CD 34, anti‐ I g M , anti‐ T d T , anti‐ HLA ‐ D r, and anti‐human kappa and lambda light chains. Additional data, such as patients' age and gender, clinical and laboratory findings such as presence of tumor masses, lymphadenopathy, hepatomegaly, splenomegaly, leukemic infiltration in the central nervous system ( CNS ) were also investigated. Results showed that 56.7% of the cases were B ‐lineage ALL and 55% were T ‐cell ALL . Also, we found that males were more affected by the disease, regardless of immunological classification. The correlation between age and immunological subtypes showed that the B ‐lineage ALL occurred more frequently in patients aged under 15while the T ‐cell ALL subtype was more frequent in adults. Immunophenotypic profiles and morphological subtypes showed a direct correlation between L3 subtype and B ‐lineage ALL , while L 1 and L 2 subtypes correlated more often with B ‐cell lineage and T ‐cell ALL , respectively. Correlation analysis between immunophenotypic and clinical profiles showed that T ‐cell ALL was more associated with a higher incidence of lymphadenopathy, hepatomegaly, splenomegaly and CNS leukemic infiltration, also showing a greater blast cell count in peripheral blood than the other subgroups. The presented data suggest that immunophenotyping is an important method in the diagnosis, monitoring and prognostic assessment in determining the pathological mechanisms of evolution of ALL .