Open AccessStudy of Hepatitis B Prevalence in Parallel With the Most Frequent HBV Genotype in South I ranian Blood Donors
Author(s) -
Karimabad Mojgan Noroozi,
Hassanshahi Gholamhossein,
Gharebaghiyan Ahmad,
Sharifi Zohre
Publication year - 2012
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21534
Subject(s) - genotype , virology , hepatitis b , hepatitis b virus , immunology , medicine , biology , genetics , gene , virus
Background Hepatitis B virus ( HBV ) is one of the leading causes of acute and chronic liver diseases and in turn responsible for a million of worldwide annual deaths. HBV has been classified into eight main groups, designated as A – H with different geographical distribution. Some genotypes are associated with different clinical outcomes and particular viral mutations. Current study was aimed to investigate the genotype and prevalence of HBV in blood donors of south of I ran. Methods This experimental study investigated the prevalence of HBV positivity in 198,289 I ranian blood donors from south of I ran by both ELISA ‐ and PCR ‐based methods. Within 198,289 donors, 120 HB s A g + cases were selected, HBV‐DNA was extracted, and the p gene sequences were amplified by nested‐ PCR . The HBV genotypes were determined by direct sequencing of the polymerase gene of HBV . Phylogenetic trees also were constructed by the neighbor‐joining ( NJ ) method. Results Findings of this study indicated that 0.184%, 0.329%, and 0.215% of blood donors were HB s A g + among I sfahan, K erman, and Y azd provinces, respectively. Only 69 (57.5%) cases of 120 HB s A g + donors were HBV‐DNA + . Sequencing analysis revealed that all of HBV ‐infected donors had the D genotype of HBV . Conclusions In conclusion, the prevalence of genotype D was 100% in I ranian HBV blood donors. These findings may have an impact on the immunological and genetic diagnosis of HBV , selection of diagnostic kits, and viral quality control panels to evaluate diagnostic methods.