Open AccessM ycobacterium wolinskyi Infection Confirmed by rpo B Gene Sequencing
Author(s) -
Jeong Ji Hun,
Seo YielHea,
Kim KyungHee,
Ahn JeongYeal,
Park PilHwan,
Park YoungKil
Publication year - 2012
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21526
Subject(s) - rpob , biology , identification (biology) , gene , dna sequencing , genetics , computational biology , rna , 16s ribosomal rna , botany
Identification of rapidly growing mycobacteria ( RGM ) is problematic because there are many taxonomic changes. 16 S r RNA gene is commonly used to identify M ycobacterium species, but alternative gene targets have been introduced for more accurate identification. We report a rare case of a prosthetic knee infection due to M ycobacterium wolinskyi . The isolate was not identified by 16 S r RNA gene sequencing alone and substantially confirmed by rpo B gene sequencing. The identification was delayed because our laboratory did not routinely identify RGM to the species level. Simultaneous sequencing of both 16 S r RNA and rpo B genes will allow rapid and accurate identification of M . wolinskyi isolates. J. Clin. Lab. Anal. 26:325‐327, 2012. © 2012 Wiley Periodicals, Inc.