Open AccessEffect of Prolonged Storage of Seminal Plasma on Levels of Calcium and Magnesium
Author(s) -
Valsa J.,
Skandhan K. P.,
Sumangala B.,
Sahab Khan P.,
Amith S.
Publication year - 2012
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21523
Subject(s) - magnesium , calcium , polyethylene , atomic absorption spectroscopy , chemistry , semen , plasma , materials science , metallurgy , andrology , medicine , physics , organic chemistry , quantum mechanics
Background In laboratories, the semen or seminal plasma samples are stored before estimation of different elements without knowing the adverse effect of storing. The present study shows the levels of Ca ++ and Mg ++ in the seminal plasma before and after the storage. Methods Atomic absorption spectrophotometry ( AAS ) was used for estimating calcium and magnesium levels in the seminal plasma. Samples were stored for one year in different containers such as polyethylene, plastic, ordinary glass, and borosil glass. Calcium and magnesium levels were estimated after this period by AAS . The total area in contact with containers was considered in the final calculation. Results After 1‐year storage, the levels of calcium and magnesium had changed. The most suitable container for storage of calcium was ordinary glass (P > 0.05) whereas ordinary glass (P > 0.05), polyethylene (P > 0.05), and borosil glass (P > 0.05) are the most suitable for magnesium. Conclusion In any elemental study, the most suitable containers are recommended for storage of the semen/seminal plasma samples. J. Clin. Lab. Anal. 26:315‐316, 2012. © 2012 Wiley Periodicals, Inc.