Open AccessPlasma Amyloid‐ Beta Peptides in Acute Cerebral Ischemia: A Pilot Study
Author(s) -
Bibl Mirko,
Welge Volker,
Schmidt Holger,
Esselmann Hermann,
Mollenhauer Brit,
Lewczuk Piotr,
Otto Markus,
Kornhuber Johannes,
Wiltfang Jens
Publication year - 2012
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21511
Subject(s) - amyloid beta , amyloid (mycology) , ischemia , beta (programming language) , chemistry , medicine , peptide , biochemistry , pathology , computer science , programming language
Background Blood‐based tests for a rapid and valid diagnosis as well as outcome prognosis of acute stroke are desirable. Recently, plasma Aβ 40 was suggested as an independent cerebrovascular risk factor candidate. Methods We investigated eight plasma samples of patients with clinical signs of acute cerebral ischemia for derangements of plasma amyloid‐beta ( Aβ ) peptide patterns as compared to 13 patients with other neuropsychiatric diseases. For the analysis of plasma, we used immunoprecipitation followed by the quantitative Aβ ‐ SDS ‐ PAGE /immunoblot. Results The major outcome was a striking decrease of Aβ 1–40 in plasma paralleled by an increase in the ratio of Aβ 1–38/ Aβ 1–40 in two patients with acute stroke. Interestingly, these patients had an onset of symptoms within only 2–4 hr before venous puncture and there was a strong correlation of Aβ 1–38/ Aβ 1–40 levels with the time span between onset of symptoms and venous puncture. Conclusion From these results, we suggest the ratio of plasma Aβ 1–38/ Aβ 1–40 as a possible biomarker for the early diagnosis of acute stroke. J. Clin. Lab. Anal. 26:238‐245, 2012. © 2012 Wiley Periodicals, Inc.