A Spectrophotometric Method to Precisely Determine Endpoint Titers in Complement Fixation Assays | Zendy

Welch Ryan J. | Zendy; Merrigan Stephen D. | Zendy; Delgado Julio C. | Zendy

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A Spectrophotometric Method to Precisely Determine Endpoint Titers in Complement Fixation Assays

Author(s) -

Welch Ryan J.,

Merrigan Stephen D.,

Delgado Julio C.

Publication year - 2012

Publication title -

journal of clinical laboratory analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.536

H-Index - 50

eISSN - 1098-2825

pISSN - 0887-8013

DOI - 10.1002/jcla.21509

Subject(s) - titer , complement fixation test , lysis , complement (music) , fixation (population genetics) , immunology , biology , biochemistry , antibody , serology , complementation , gene , phenotype

Since the early 20th century, complement fixation (CF) testing has been used to quantify the humoral response to various pathogens. The qualification of a positive result is based on a subjective determination of 30% lysis of sheep red blood cells, which can lead to variability in the analysis. A spectrophotometric reading of a standard with a known 30% lysis was used to standardize the currently used CF method and tested with controls and patient sera for various fungal assays. By utilizing this method a precise and non‐subjective determination of endpoint titers was achieved. J. Clin. Lab. Anal. 26:190‐193, 2012. © 2012 Wiley Periodicals, Inc.

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