Open AccessFactor V L eiden, Prothrombin 20210 G > A , MTHFR 677 C > T and 1298 A > C , and Homocysteinemia in T unisian Blood Donors
Author(s) -
Hadhri Samira,
Rejab Mohamed Ben,
Guedria Hajer,
Ifa Lamia,
Chatti Noureddine,
Skouri Hadef
Publication year - 2012
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21506
Subject(s) - methylenetetrahydrofolate reductase , genotype , factor v leiden , microbiology and biotechnology , population , allele , medicine , genetics , biology , venous thrombosis , thrombosis , gene , environmental health
Specific genetic conditions are known to be associated with high risk of venous thromboembolism. This genetic basis varies widely between ethnic groups. We investigated the distribution of four inherited polymorphisms in 113 unselected T unisian blood donors by using polymerase chain reaction‐restriction fragment length polymorphism ( PCR ‐ RFLP ) method. The allele frequencies of Factor V L eiden ( FVL ), prothrombin 20210G> A , methylenetetrahydrofolate reductase ( MTHFR ) 677 C > T , and MTHFR 1298 A > C mutations were 3, 0.9, 30, and 31%, respectively. The MTHFR 677 C > T polymorphism was influenced by age. Twenty‐nine of the 113 blood donors demonstrated more than one genetic markers. Hyperhomocysteinemia was found in 12 subjects, and it was statistically associated to the MTHFR 677 TT genotype. Principal component analysis allowed disclosing the resemblance between M editerranean populations. Our findings may be helpful for population genetics study, and provide epidemiologic database for further studies in thrombosis field among T unisians. J. Clin. Lab. Anal. 26:167‐173, 2012. © 2012 Wiley Periodicals, Inc.