Open AccessSerum Creatinine Determined by Jaffe, Enzymatic Method, and Isotope Dilution‐Liquid Chromatography‐Mass Spectrometry in Patients Under Hemodialysis
Author(s) -
Liu WenSheng,
Chung YuTing,
Yang ChihYu,
Lin ChihChing,
Tsai KunHung,
Yang WuChang,
Chen TzenWen,
Lai YenTing,
Li SzuYuan,
Liu TsungYun
Publication year - 2012
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.21495
Subject(s) - creatinine , chemistry , chromatography , isotope dilution , albumin , hemodialysis , globulin , medicine , mass spectrometry , biochemistry
Objectives Serum creatinine is an important clinical marker for renal clearance. However, the Jaffe method had much interference and the accuracy had not been tested in patients under hemodialysis ( HD ) with standard isotope dilution‐liquid chromatography‐mass spectrometry ( IDLCMS ) method. The validity of enzymatic method is also unknown. Methods The predialysis serum creatinine levels of 126 patients under regular HD for 3 months were checked by J affe, enzymatic, and IDLCMS methods. We compared the value of the J affe and enzymatic to that of IDLCMS in linear regression model. And we also tried to find the clinical parameters that influence the difference between J affe vs. IDLCMS and enzymatic vs. IDLCMS method. Results We found significant underestimate serum creatinine in uremic patients by J affe and enzymatic methods. Serum glucose and globulin are positive biases, whereas albumin, potassium, and phosphorus are negative biases. Enzymatic method is less affected by serum glucose and serum protein. Albumin acts differently in uremic serum compared to the results of mixing them with normal serum. Conclusions For uremic patients, in whom creatinine level is high and many of them suffered from diabetes mellitus, serum creatinine can be either under‐ or overestimated by Jaffe method. Enzymatic method is less affected and may be a better method. J. Clin. Lab. Anal. 26:206‐214, 2012. © 2012 Wiley Periodicals, Inc.