Open AccessDetection of Staphylococcus aureus with a fluorescence in situ hybridization that does not require lysostaphin
Author(s) -
Lawson Thomas S.,
Connally Russell E.,
Iredell Jonathan R.,
Vemulpad Subramanyam,
Piper James A.
Publication year - 2011
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20448
Subject(s) - lysostaphin , lysozyme , staphylococcus aureus , fluorescence in situ hybridization , microbiology and biotechnology , biology , chemistry , bacteria , biochemistry , gene , genetics , chromosome
To detect with whole‐cell fluorescence in situ hybridization (FISH), Staphylococcus aureus is typically permeabilized with lyozyme and lysostaphin. We tested whether it was feasible to detect S. aureus and differentiate it from Staphylococcus epidermidis with lysozyme‐only permeabilization. We compared lysozyme permeabilizationto S. aureus permeabilized with lysozyme in combination with lysostaphin. It was determined that S. aureus treated with agarose, methanol, and lysozyme could be detected with FISH. The 1 hr protocol is a useful alternative to conventional FISH. J. Clin. Lab. Anal. 25:142–147, 2011. © 2011 Wiley‐Liss, Inc.