Open AccessImmunoenzymatic assay for the diagnosis of American tegumentary leishmaniasis using soluble and membrane‐enriched fractions from infectious Leishmania ( Viannia ) braziliensis
Author(s) -
Cataldo Jamyra Iglesias,
de Queiroz Mello Fernanda Carvalho,
MoutaConfort Eliame,
de Fátima Madeira Maria,
de Oliveira Schubach Armando,
da Silva Genestra Marcelo,
Ribeiro Flávia Coelho,
de Fátima MoreiraVenâncio Célia,
Passos Sônia Regina Lambert
Publication year - 2010
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20402
Subject(s) - leishmania , antigen , parasite hosting , leishmaniasis , medicine , virology , biology , immunology , world wide web , computer science
Abstract The diagnosis of American tegumentary leishmaniasis (ATL) is based on the visualization or isolation of the parasite, which is a time‐consuming and poorly sensitive method. In this study, we evaluated the accuracy and reliability of ELISA for the diagnosis of ATL using soluble (SF) and membrane‐enriched (MF) antigen fractions obtained from an infectious strain of Leishmania ( Viannia ) braziliensis . A total of 152 serum samples investigated at a referral center in Rio de Janeiro, Brazil, between 2005 and 2007 were studied. Each sample was tested twice with each fraction for the calculation of reliability (intraclass coefficient (ICC)). Cut‐off values of 0.22 (SF) and 0.33 (MF) were defined. The use of the fractions resulted in good discrimination between patients, with a large area under the curve ( P <0.0001), but no difference was observed between the two fractions ( P =0.45). Sensitivity was 89.5% for each fraction, specificity was 89.5% for SF and 93.4% for MF, and the positive likelihood ratio was 8.5 for SF and 13.6 for MF. The ICCs were excellent (SF: 0.96 and MF: 0.90). The antigens tested provided precision and accuracy for the diagnosis of ATL, with SF being recommended due to its lower cost and greater practicality. J. Clin. Lab. Anal. 24:289–294, 2010. © 2010 Wiley‐Liss, Inc.