Open AccessDirect diagnosis of Leishmania species on serosity materials punctured from cutaneous leishmaniasis patients using PCR‐RFLP
Author(s) -
Hajjaran Homa,
Vasigheh Farzaneh,
Mohebali Mehdi,
Rezaei Sasan,
Mamishi Setareh,
Charedar Soroure
Publication year - 2011
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20377
Subject(s) - restriction fragment length polymorphism , cutaneous leishmaniasis , leishmania , polymerase chain reaction , leishmaniasis , leishmania major , biology , leishmania tropica , microbiology and biotechnology , parasite hosting , immunology , genetics , gene , world wide web , computer science
This study was aimed at identifying the Leishmania species using serosity materials punctured from skin lesions of cutaneous leishmaniasis (CL) patients by using internal transcribed spacer1 (ITS1) polymerase chain reaction (PCR)‐restriction fragment length polymorphism (RFLP). We used the PCR‐RFLP on 60 parasitological confirmed CL patients who referred to leishmaniasis laboratory from the School of Public Health, Tehran University of Medical Sciences. The PCR‐RFLP could correctly detect 51 Leishmania species of the 60confirmed positive specimens, where all the other 10 parasitological (microscopy and culture) negative samples that were prepared from other bacterial‐ and fungal‐infected lesions had negative results. The results also revealed that Leishmania major was the dominant species (53.3%). This study suggests that the PCR‐RFLP assay with serosity materials punctured from CL patients using Hae III enzyme is useful for the rapid identification of Leishmania species. J. Clin. Lab. Anal. 25:20–24, 2011. © 2011 Wiley‐Liss, Inc.