Open AccessSerodiagnosis of Chlamydia pneumoniae infection using three inclusion membrane proteins
Author(s) -
Hongliang Chen,
Zhou Zhou,
Zhan Hu,
Yanhua Zeng,
Zhongyu Li,
Yingbiao Lin,
Guozhi Dai,
Yimou Wu
Publication year - 2010
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20367
Subject(s) - chlamydia , microbiology and biotechnology , inclusion (mineral) , chlamydophila pneumoniae , chlamydiaceae , virology , medicine , biology , immunology , chemistry , mineralogy
The Chlamydia pneumoniae genome‐encoded open reading frames Cpn0146, Cpn0147, and Cpn0308 were expressed as recombinant proteins for detecting C. pneumoniae ‐specific antibodies in samples from three groups of individuals including 183 with C. pneumoniae ‐associated respiratory infection (group I), 60 healthy blood donors (group II), and 32 with no known respiratory infection (group III). The recombinant Cpn0146 was recognized by 71 (38.8% positive recognition rate), 15 (25%) and 1 (3.1%), Cpn0147 by 75 (40.9%), 14 (23.3%), and 2 (6.3%), and Cpn0308 by 82 (44.8%), 16 (26.7%), and 0 (0%) samples from groups I, II, and III, respectively. The positive recognition rates with any of the three antigens were significantly higher in group I than those in groups II and III, suggesting that more individuals from group I were likely infected with C. pneumoniae . This conclusion was confirmed with a commercially available whole organism‐based ELISA kit (Savyon Diagnostics Ltd., Ashdod, Israel), which detected C. pneumoniae antibodies in 98 (64.1%), 26 (43.3%), and 4 (12.5%) samples from group I, II, and III, respectively. Comparing to the commercial kit, the recombinant antigen‐based detection assays displayed >97% of detection specificity and >87% of sensitivity, suggesting that these recombinant antigens can be considered alternative tools for aiding in serodiagnosis of C. pneumoniae infection. J. Clin. Lab. Anal. 24:55–61, 2010. © 2010 Wiley‐Liss, Inc.