Open AccessLectin affinity electrophoresis of serum alkaline phosphatase in metastasized breast cancer
Author(s) -
Le Bricon Thierry,
GayBellile Cécile,
Cottu Paul,
Benlakehal Mourad,
Guillon Hélène,
Houzé Pascal
Publication year - 2010
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20357
Subject(s) - alkaline phosphatase , medicine , breast cancer , gastroenterology , lectin , cancer , pathology , endocrinology , chemistry , enzyme , immunology , biochemistry
The use of serum alkaline phosphatase (ALP) isoenzymes as markers of breast cancer metastases and treatment efficacy has received little attention. Twenty‐six breast cancer women (56±13 years, all post‐menopausal) were prospectively evaluated during their first and third course of chemotherapy (4‐week interval). Serum samples were analyzed for ALP isoenzymes (bone, liver, and intestine) using a lectin affinity electrophoresis kit (Hydragel 15 ISO‐PAL ® , Sebia) adapted on a semi‐automated Hydrasys ® system (Sebia). Results were compared with imaging techniques for the presence of metastases; bone ALP isoenzyme (B‐ALP) results werecompared with C‐Terminal degradation products of type I collagen (S‐CTX) (CrossLaps ® , IDS Nordic). Serum B‐ALP, but not S‐CTX, confirmed the presence of bone metastases (BM) ( n =15) with 67/100% sensitivity/specificity (using a 69 UI/L ROC cut‐off); ROC AUC was 0.806 ( P =0.0004) (NS for S‐CTX). Chemotherapy reduced serum B‐ALP by 24% over 4 weeks ( P =0.0012); there was no change for S‐CTX. There was no specific clinical pattern for other ALP isoenzymes (liver and intestine). In conclusion, serum B‐ALP, but not S‐CTX, could help confirm the presence of BM in breast cancer patients. J. Clin. Lab. Anal. 24:20–24, 2010. © 2010 Wiley‐Liss, Inc.