Open AccessRapid liquid chromatography/tandem mass spectrometer (LCMS) method for clozapine and its metabolite N‐desmethyl Clozapine (Norclozapine) in human serum
Author(s) -
Rao L.V.,
Snyder M.L.,
Vallaro G.M.
Publication year - 2009
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20345
Subject(s) - desmethyl , metabolite , clozapine , chromatography , chemistry , tandem , mass spectrometry , tandem mass spectrometry , medicine , schizophrenia (object oriented programming) , biochemistry , materials science , psychiatry , composite material
Clozapine is indicated for the treatment of schizophrenia and related psychotic disorders. Several methods have been developed for monitoring Clozapine levels; however, they possess limited specificity and are often laborious. This study describes a simple liquid chromatography/tandem mass spectrometer (LCMS) method in human serum. The ion transitions monitored were m / z 327, 270, 296 for Clozapine, m / z 313, 192, 227 for Norclozapine and m / z 328, 271 for Loxapine. The assay is linear (25–1000 ng/ml) and showed a good correlation ( r =0.98) within the analytical range of 79–1210 ng/ml in human serum. This assay is highly specific and sensitive for the simultaneous measurements of Clozapine and Norclozapine. The simplification of this assay makes it ideal for high throughput analyses of the patient samples in a routine clinical laboratory staffed with general medical technologists. J. Clin. Lab. Anal. 23:394–398, 2009. © 2009 Wiley‐Liss, Inc.