Open AccessDetection of Paracoccidioides brasiliensis gp43 Gene in Sputa by Loop‐Mediated Isothermal Amplification Method
Author(s) -
Tatibana B. T.,
Sano A.,
Uno J.,
Kamei K.,
Igarashi T.,
Mikami Y.,
Miyaji M.,
Nishimura K.,
Itano E. N.
Publication year - 2009
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20304
Subject(s) - paracoccidioides brasiliensis , paracoccidioidomycosis , loop mediated isothermal amplification , microbiology and biotechnology , biology , paracoccidioides , pathogen , gene , immunology , virology , genetics , dna
The fungus Paracoccidioides brasiliensis is the pathogen of paracoccidioidomycosis (PCM), a systemic mycosis prevalent in Latin America. The loop‐mediated isothermal amplification method (LAMP) was used in this study to detect the presence of P. brasiliensis in sputa samples from patients with chronic PCM, suspected PCM, and a negative control. The target P. brasiliensis gp43 gene was amplified in less than 4 hr in 11 of 18 sputa samples tested. The LAMPmethod had the advantage of speed and simplicity compared with the classic diagnostic methods such as the histopathological test or biological material culture and did not require sophisticated technical apparatus. It would be an important aid in cases where immediate treatment would mean patient survival, especially in immune‐suppressed patients. J. Clin. Lab. Anal. 23:139–143 2009. © 2009 Wiley‐Liss, Inc.