Development of an enzyme‐linked immunosorbent assay for the detection of ciguatoxin in fish tissue using chicken immunoglobulin Y | Zendy

Empey Campora Cara | Zendy; Hokama Yoshitsugi | Zendy; Yabusaki Kenichi | Zendy; Isobe Minoru | Zendy

Open Access

Development of an enzyme‐linked immunosorbent assay for the detection of ciguatoxin in fish tissue using chicken immunoglobulin Y

Author(s) -

Empey Campora Cara,

Hokama Yoshitsugi,

Yabusaki Kenichi,

Isobe Minoru

Publication year - 2008

Publication title -

journal of clinical laboratory analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.536

H-Index - 50

eISSN - 1098-2825

pISSN - 0887-8013

DOI - 10.1002/jcla.20256

Subject(s) - horseradish peroxidase , ciguatoxin , conjugate , antibody , microbiology and biotechnology , monoclonal antibody , fish <actinopterygii> , enzyme , chemistry , immunoassay , cross reactivity , biology , biochemistry , cross reactions , immunology , fishery , mathematical analysis , mathematics

A sandwich enzyme‐linked immunosorbent assay was developed to detect ciguatoxin (CTX) in fish tissue. The assay utilizes two antibodies, chicken immunoglobulin Y specific to the ABCD domain of CTX and a mouse monoclonal immunoglobulin G–hor‐seradish peroxidase conjugate specific to the JKLM domain of CTX. The sensitivity, working range, cross reactivity, accuracy, precision, and reproducibility were examined. J. Clin. Lab. Anal. 22:239–245, 2008. © 2008 Wiley‐Liss, Inc.

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