Open AccessDevelopment of an enzyme‐linked immunosorbent assay for the detection of ciguatoxin in fish tissue using chicken immunoglobulin Y
Author(s) -
Empey Campora Cara,
Hokama Yoshitsugi,
Yabusaki Kenichi,
Isobe Minoru
Publication year - 2008
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20256
Subject(s) - horseradish peroxidase , ciguatoxin , conjugate , antibody , microbiology and biotechnology , monoclonal antibody , fish <actinopterygii> , enzyme , chemistry , immunoassay , cross reactivity , biology , biochemistry , cross reactions , immunology , fishery , mathematical analysis , mathematics
A sandwich enzyme‐linked immunosorbent assay was developed to detect ciguatoxin (CTX) in fish tissue. The assay utilizes two antibodies, chicken immunoglobulin Y specific to the ABCD domain of CTX and a mouse monoclonal immunoglobulin G–hor‐seradish peroxidase conjugate specific to the JKLM domain of CTX. The sensitivity, working range, cross reactivity, accuracy, precision, and reproducibility were examined. J. Clin. Lab. Anal. 22:239–245, 2008. © 2008 Wiley‐Liss, Inc.