Open AccessDetermination of terephthalic acid isopropylamide in urine with a liquid chromatography/mass spectrometry (LC/MS) method
Author(s) -
Baumann F.,
MauzKörholz C.,
Clauß D.,
Borrmann S.,
Giannis A.,
Merkel N.,
Körholz D.,
Preiss R.
Publication year - 2008
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20213
Subject(s) - chromatography , chemistry , terephthalic acid , mass spectrometry , urine , liquid chromatography–mass spectrometry , detection limit , metabolite , solid phase extraction , extraction (chemistry) , selected reaction monitoring , gas chromatography–mass spectrometry , tandem mass spectrometry , organic chemistry , biochemistry , polyester
Abstract A sensitive and simple liquid chromatography/mass spectrometry (LC/MS) method was developed for the determination of terephthalic acid isopropylamide, the final metabolite of procarbazine in human urine. A solid‐phase extraction with C 18 cartridges was used followed by LC/MS with a single mass spectrometer (SSQ 7000 from Finnigan). Terephthalic acid isobutylamide was the internal standard. The quantification limit was 30 ng/mL in urine (6 × noise). This assay was applied for drug monitoring of terephthalic acid isopropylamide in urine after oral administration of procarbazine in children and adolescents with Hodgkin lymphomas. J. Clin. Lab. Anal. 22:21–28, 2008. © 2008 Wiley‐Liss, Inc.