Open AccessCellular immune response from chagasic patients to CRA or FRA recombinant antigens of Trypanosoma cruzi
Author(s) -
Lorena Virginia M.B.,
Verçosa Alinne F.A.,
Machado Raquel C.A.,
MoitinhoSilva Lucas,
Cavalcanti Maria G.A.,
Silva Edimilson D.,
Ferreira Antonio G.P.,
CorreaOliveira Rodrigo,
Pereira Valéria R.A.,
Gomes Yara M.
Publication year - 2008
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20209
Subject(s) - antigen , immune system , trypanosoma cruzi , peripheral blood mononuclear cell , immunology , chagas disease , cytokine , tumor necrosis factor alpha , biology , recombinant dna , stimulation , interferon gamma , in vitro , microbiology and biotechnology , endocrinology , parasite hosting , biochemistry , world wide web , computer science , gene
We propose to analyze the relation between the cellular immune response of Chagas' disease patients after in vitro stimulation of peripheral blood mononuclear cells (PBMC) with recombinant antigens cytoplasmatic repetitive antigen (CRA) or flagellar repetitive antigen (FRA) of T. cruzi and the chronic clinical forms of disease. Cells were stimulated using phytohemagglutinin, CRA, FRA, or a soluble antigen of Epimastigota (Ag‐Epi) for 24 hr, 72 hr, or 6 days. The proliferation of cells was evaluated after 6 days of culture by quantification of incorporated 3 H‐thymidine. Cytokines were measured in the supernatants obtained after 24 hr (tumor necrosis factor [TNF]‐α and interleukin [IL]‐4), 72 hr (IL‐10), and 6 days (interferon [IFN]‐γ) using enzyme‐linked immunosorbent assay (ELISA). Cells of the Chagas patients stimulated with the recombinant antigens exhibited higher proliferation responses compared with that of non‐Chagas (NC) individuals. However, when proliferation was compared between patients with the cardiac form (CF) or indeterminate form (IF), it was not possible to establish a difference in the response. So far as the cytokines secreted in the culture supernatants after stimulation in vitro with T. cruzi antigens were concerned, the results showed that CRA, as well as Epi‐Ag, were able to stimulate the production of TNF‐α and IFN‐γ in Chagas patients as compared with NC individuals. However, the cytokine levels after stimulation with the T. cruzi antigens were not different between the patients with CF and IF. CRA was capable of inducing a T helper type 1 (Th1) immune response, with elevated production of TNF‐α and IFN‐γ in Chagas patients that are carriers of CF and IF clinical forms. J. Clin. Lab. Anal. 22:91–98, 2008. © 2008 Wiley‐Liss, Inc.