Open AccessImproved method to calculate the antibody avidity index
Author(s) -
Perciani C.T.,
Peixoto P.S.,
Dias W.O.,
Kubrusly F.S.,
Tanizaki M.M.
Publication year - 2007
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20172
Subject(s) - avidity , titration , index (typography) , titration curve , value (mathematics) , point (geometry) , antibody , mathematics , statistics , computer science , chemistry , immunology , medicine , geometry , inorganic chemistry , world wide web
The methods currently used to determine the immunoglobulin avidity index (AI) require the choice of a reference point in the ELISA titration curve. Since both curves, with and without denaturating reagents, seldom run in parallel, the AI value becomes highly dependent on this reference. The new method for AI calculation presented here takes into account the whole data of the ELISA titration curve in which the final numerical AI is the average of each point. J. Clin. Lab. Anal. 21:201–206, 2007. © 2007 Wiley‐Liss, Inc.