Open AccessSpectrofluorimetric determination of bile acid using a europium‐doxycycline probe
Author(s) -
Wang Ting,
Wang Xiaolei,
Jiang Chongqiu
Publication year - 2007
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20137
Subject(s) - europium , doxycycline , chemistry , bile acid , chromatography , biochemistry , antibiotics , organic chemistry , ion
A new spectrofluorimetric method was developed for the determination of trace amounts of bile acid (BA). Using europium ion (Eu 3+ )‐doxycycline (DC) as a fluorescent probe, in a buffer solution of pH=7.0, BA can remarkably reduce the fluorescence intensity of the DC‐Eu 3+ complex at λ=612 nm; the reduced fluorescence intensity of the Eu 3+ is proportional to the concentration of BA. Optimum conditions for the determination of BA were also investigated. The linear range and detection limit for the determination of BA were 5.0 × 10 −8 mol/L to 5.5 × 10 −7 mol/L and 1.1 × 10 −8 mol/L, respectively. This method is practical and relatively free of interference from coexisting substances, and can be successfully applied to assess BA in serum samples. J. Clin. Lab. Anal. 21:207–212, 2007. © 2007 Wiley‐Liss, Inc.