Open AccessRole of enzyme‐treated cells in RBC antibody screening using the gel test: a study of anti‐RH1, ‐RH2, and ‐RH3 antibodies
Author(s) -
Conne Jocelyne,
Schneider Philippe,
Tissot JeanDaniel
Publication year - 2007
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20120
Subject(s) - antibody , antibody titer , titer , microbiology and biotechnology , chemistry , immunology , chromatography , medicine , biology
The role of enzyme‐treated cells (ETCs) in red blood cell (RBC) antibody screening has been the subject of controversy, and its place in the clinical routine remains to be determined. In this work, plasma samples containing anti‐RH1 (anti‐D; N = 10), anti‐RH2 (anti‐C; N = 10), or anti‐RH3 (anti‐E; N = 10) antibodies were studied. The samples were diluted in nonbuffered or buffered normal saline, as well as in a pool of AB plasma samples. Titers and scores were determined by means of the gel test, using the indirect antiglobulin test (IAT) as well as ETCs, with R 0 r, r′r, or r″r test cells. Our results showed that compared to the IAT, ETCs allowed a clearer detection of anti‐RH2 and anti‐RH3, but not of anti‐RH1 antibodies. Based on our study, it is not clear whether the ETC phase of the gel test should be maintained for RBC antibody screening. J. Clin. Lab. Anal. 21:61–66, 2007. © 2007 Wiley‐Liss, Inc.