Open AccessStudy of V1a vasopressin receptor gene single nucleotide polymorphisms in platelet vasopressin responsiveness
Author(s) -
Hasan Kazi N.,
Shoji Masaru,
Tsutaya Shoji,
Kudo Ryoko,
Matsuda Eriko,
Saito Junko,
Kimura Tokihisa,
Yasujima Minoru
Publication year - 2006
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20106
Subject(s) - single nucleotide polymorphism , vasopressin , genotype , allele , polymorphism (computer science) , biology , gene , restriction fragment length polymorphism , promoter , genetics , polymerase chain reaction , vasopressin receptor , microbiology and biotechnology , medicine , receptor , gene expression , antagonist
There is a significant heterogeneity among individuals in terms of platelet aggregation response to arginine vasopressin (AVP). The aim of this study was to evaluate whether four single nucleotide polymorphisms (SNPs) in the promoter region of vasopressin V1a receptor gene ( V1aR ) could be used as genetic markers for divergent platelet aggregation response to AVP. Seventeen of 33 subjects showed more than 60% of maximum platelet aggregation and were classified as responders. Sixteen were classified as nonresponders because they had less than 30% aggregation. In a preliminary study, V1aR gene sequences were determined in two responders and two nonresponders. We found four SNPs in the promoter region of the V1aR gene: –6951G/A, –4112A/T, –3860T/C, and –242C/T. In all 33 subjects the genotypes of four SNPs were determined using either polymerase chain reaction (PCR) with allele‐specific primers or PCR followed by restriction‐fragment length polymorphism (RFLP). There were no differences in the AVP‐induced aggregation between the subjects with and without variant alleles of each four SNPs. The genotype frequencies of four SNPs of V1aR were almost identical between AVP responders and nonresponders. These results suggest that the four SNPs in the promoter region of the V1aR gene may not be useful as genetic markers for platelet aggregation heterogeneity. J. Clin. Lab. Anal. 20:87–92, 2006. © 2006 Wiley‐Liss, Inc.