Open AccessPresence of immunounreactive albumin in the urine of diabetic patients
Author(s) -
Nakayama Aki,
Ida Takashi,
Hatano Yoshiji,
Oohara Kayoko,
Yoshida Hiroshi,
Baba Toshiaki,
Sakamoto Hisashi,
Shiba Kiyoko
Publication year - 2006
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20100
Subject(s) - chromatography , high performance liquid chromatography , albumin , urine , chemistry , immunoassay , dipstick , sodium dodecyl sulfate , polyacrylamide gel electrophoresis , medicine , biochemistry , immunology , enzyme , antibody
Recent studies have demonstrated that conventional immunochemical assays underestimate urinary albumin concentration because of the presence of immunounreactive albumin. It has been reported that intact urinary albumin in 24‐hr diabetic urine samples could be detected as total concentration (immunoreactive+immunounreactive) by an HPLC method based on size exclusion chromatography. The aim of this study was to investigate urinary albumin concentration in diabetic spot urine samples by comparing the HPLC method with several other methods. The albumin concentrations on 80 diabetic spot urine specimens were measured by turbidimetric immunoassay (TIA), high performance liquid chromatography (HPLC), and a dipstick method.In addition, they were also analyzed by reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and native polyacrylamide gel electrophoresis (Native PAGE). The albumin concentrations derived from diabetic spot urine samples measured by the HPLC method were higher than those of the other methods except for five of 80 samples. Furthermore, the albumin concentrations analyzed by Native PAGE were higher than SDS PAGE in 61 (76.2%) of 80 samples. This study suggests the need for evaluating diabetes not only by HPLC, but also by combining it with another method. J. Clin. Lab. Anal. 20:29–36, 2006. © 2006 Wiley‐Liss, Inc.