Open AccessDiagnosis of Chlamydia trachomatis infection
Author(s) -
ManiaPramanik Jayanti,
Potdar Shobha,
Kerkar Shilpa
Publication year - 2006
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20092
Subject(s) - chlamydia trachomatis , ligase chain reaction , polymerase chain reaction , chlamydia trachomatis infection , nucleic acid amplification tests , infection rate , population , chlamydia , virology , medicine , computational biology , biology , immunology , surgery , multiplex polymerase chain reaction , environmental health , genetics , gene
Important progress in the diagnosis of Chlamydia trachomatis ( C. trachomatis ) includes the development of nucleic acid amplification techniques such as polymerase chain reaction (PCR) and ligase chain reaction (LCR). Commercial kits are available, but they are costly, sporadic in availability, must be imported, and are economically beyond the reach of common people. To overcome this limitation, most research laboratories have standardized their in‐house‐developed PCR methods for diagnosing this infection. However, each laboratory has to spend a great deal of time and money to accomplish this. Published reports do not always elaborate the steps involved in standardizing a test so that it can immediately be reproduced in another setting. In the present study we attempted to elaborate the steps involved in standardizing a sensitive and specific PCR technique followed by hybridization with specific C. trachomatis probe to diagnose this infection in cervical, introital, and urine specimens, and used it to determine the infection rate in a clinical population. J. Clin. Lab. Anal. 20:8–14, 2006. © 2006 Wiley‐Liss, Inc.