Open AccessSurface‐enhanced laser desorption/ionization‐time of flight‐mass spectrometry (SELDI‐TOF‐MS): A new proteomic urinary test for patients with urolithiasis
Author(s) -
Cadieux Peter A.,
Beiko Darren T.,
Watterson James D.,
Burton Jeremy P.,
Howard Jeffrey C.,
Knudsen Bodo E.,
Gan Bing Siang,
McCormick John K.,
Chambers Ann F.,
Denstedt John D.,
Reid Gregor
Publication year - 2004
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20018
Subject(s) - surface enhanced laser desorption/ionization , mass spectrometry , time of flight mass spectrometry , chemistry , desorption , chromatography , urinary system , ionization , analytical chemistry (journal) , protein mass spectrometry , medicine , electrospray ionization , ion , adsorption , organic chemistry
SELDI‐TOF‐MS is a highly sensitive protein‐analysis tool capable of detecting minute protein profile differences between biological samples. As proteins have been associated with urinary tract calculi, protein‐based urinalysis may offer insights into their diagnosis. The purpose of this study was to evaluate SELDI‐TOF‐MS as a potential method for identifying urinary biomarkers of urolithiasis. Midstream sterile urine samples were obtained from 25 male patients with a confirmed diagnosis of urolithiasis (test group) and 25 male subjects with no known history of the disease (controls). Urinary levels of oxalate, total protein, albumin, and osteopontin were determined. Protein profiles were generated using SELDI‐TOF‐MS. SELDI‐TOF‐MS profiling revealed a relationship between protein peak intensities at 67 and 24 kDa that differed between the two groups. The ratio of p67:p24 was found to be less than 1.0 in all of the control samples (mean 0.26), while 18 out of 25 (72%) of the test group samples displayed a ratio greater than 1.0 (total group mean 4.75, P <0.001). Albumin, total protein, and oxalate levels were higher in the test group than the controls. Although SELDI‐TOF‐MS is not yet in widespread use in hospital and diagnostic laboratories, this system represents a promising new method for rapidly identifying patients with urolithiasis. J. Clin. Lab. Anal. 18:170–175, 2004. © 2004 Wiley‐Liss, Inc.