Open AccessEvaluation of a modified IAT‐gel with polyethylenglycol (PEG) addition method for red blood cell antibody identification
Author(s) -
Gustafsson L.,
Wikman A.,
Lundahl J.
Publication year - 2004
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.20017
Subject(s) - antibody , peg ratio , red blood cell , chemistry , enzyme , chromatography , microbiology and biotechnology , biochemistry , immunology , medicine , biology , finance , economics
We aimed to evaluate whether a PEG‐IAT method, modified for gel technique, could replace the two‐stage enzyme method for routine identification of red blood cell (RBC) antibodies. A total of 161 selected patient samples were analyzed with the two‐stage enzyme tube technique and the PEG‐IAT in gel techniques. In 65 samples, identical antibody results were obtained by the two methods. An additional 28 antibodies were identified by either the PEG‐IAT gel method or the enzyme method. Twenty antibodies were identified by the PEG‐IAT gel method only (12 Rh antibodies, four anti‐Kell, two anti‐Fya, two anti‐Jka), and eight antibodies by the enzyme method only (four Rh antibodies, one anti‐K, one anti‐Jka, two anti‐Lea). The results in the remaining 68 samples were judged as nonspecific reactions, which were more common when the enzyme method was used. We concluded that the PEG‐IAT in gel method identifies more RBC antibodies with less nonspecific reactions than the two‐stage enzyme technique. However, no single method detected all clinical significant RBC antibodies. J. Clin. Lab. Anal. 18:165–169, 2004. © 2004 Wiley‐Liss, Inc.