Open AccessDna extraction and amplification from giemsa‐stained blood smears
Author(s) -
Yokota Masaharu,
Tatsumi Noriyuki,
Tsuda Izumi,
Yano Ikuya
Publication year - 1995
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860090609
Subject(s) - giemsa stain , dna extraction , dna , polymerase chain reaction , microbiology and biotechnology , blood smear , blood stains , biology , staining , pathology , gene , genetics , medicine , immunology , malaria
DNA extraction was attempted from Giemsa‐stained blood smears on glass slides that had been stored for several years. High molecular weight DNA bands were clearly visible after electrophoresis when DNA was extracted from specimens stored up to 2 years. Specimens more than 4 years old demonstrated a smeared pattern, suggesting degeneration of the DNA, but they could be rescued by PCR amplification using primers of HLA‐DQA1 genes. The recovery could be pursued even in 11‐year‐old specimens.