Open AccessCurrent status of available standards for quality improvement of assays for detection of autoantibodies to nuclear and intracellular antigens
Author(s) -
Nakamura Robert M.,
Bylund David J.,
Tan Eng M.
Publication year - 1994
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860080605
Subject(s) - autoantibody , antigen , standardization , immunology , extractable nuclear antigens , medicine , intracellular , disease , antibody , computational biology , biology , computer science , pathology , biochemistry , anti nuclear antibody , operating system
Considerable progress has been made in the improvement of clinical assays for the detection of autoantibodies to nuclear and intracellular antigens with the use of available World Health Organization (WHO) and Arthritis Foundation/Centers for Disease Control (AF/CDC) standards. The ultimate goal of standardization is for various clinical laboratory test results to be interchangeable and for an exchange of data to be done with confidence. This report discusses the available standards. In addition, significant technical problems and variations in methodologies for the detection of autoantibodies to intracellular antigens noted during a 4‐year study by a European Consensus Study Group are detailed. Currently, there is a need for a future generation of reference preparations and standards that will show specific antibody reactivity on sensitive enzymes and immunoblotting assays. Standardization efforts should be done to characterize specific nuclear and cellular antigen preparations that may be of natural or of recombinant technology origin. © 1994 Wiley‐Liss, Inc.