Open AccessPrimed in situ DNA amplification (PIDA)
Author(s) -
Moss Ronald B.,
Kaliner Michael A.
Publication year - 1994
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860080211
Subject(s) - microbiology and biotechnology , oligonucleotide , in situ , primer (cosmetics) , dna , dig , in situ hybridization , biology , nucleotide , chemistry , genetics , gene , messenger rna , organic chemistry
A novel PCR in situ protocol is described which can be implemented in one day. An initial amplification step with a single primer pair is followed by an amplification with an internal oligonucleotide probe with labeled nucleotides (dig‐dUTP). Detection is then accomplished with an antidigoxigenin antibody and substrate solution. This rapid and sensitive method may be useful in diagnosing low copy DNA in tissue specimens and cells. © 1994 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.