Open AccessVariable‐region subgroup distribution among λ‐type immunoglobulins in normal human serum
Author(s) -
Abe Masahiro,
Ozaki Shuji,
Wolfenbarger Dennis,
DebramHart Mary,
Weiss Deborah T.,
Solomon Alan
Publication year - 1994
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860080103
Subject(s) - antibody , multiple myeloma , microbiology and biotechnology , population , waldenstrom macroglobulinemia , medicine , immunology , chemistry , biology , lymphoma , environmental health
The distribution of the major V L subgroups (V λI , V λII , V λIII , V λIV , V λVI , and V λVIII ) among λ‐type immunoglobulins (Igs) in normal serum was determined by a sandwich enzymelinked immunosorbent assay (ELISA) using a panel of murine anti‐human V λ ‐subgroup‐specific monoclonal antibodies (MoAbs) and appropriate reference standard proteins. The mean concentration of λI, λII, λIII, λIV, λVI, and λVIII Igs in serum specimens obtained from 23 adults was 2158, 162, 1958, 264, 225, and 169 μg/mL and represented 44, 3, 40, 5, 5, and 3% of the total Igλ population, respectively. The low percentage of λII Igs in normal serum was in marked contrast to the ∼40% incidence of this V λ subgroup found among λ‐type Bence Jones proteins and monoclonal serum Igs obtained from patients with multiple myeloma and AL amyloidosis, or ∼60% in Waldenström's macroglobulinemia. The non‐random expresson of the V λ II subgroup in these diseases implies a relationship between V λ ‐gene usage and plasma cell, as well as certain types of lymphocytic dyscrasias. © 1994 Wiley‐Liss, Inc.