Open AccessProduction of hydrogen peroxide by neutrophilic polymorphonuclear leukocytes in patients with diabetic nephropathy
Author(s) -
Watanabe Asako,
Tomino Yasuhiko,
Yokoyama KenIchi,
Koide Hikaru
Publication year - 1993
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860070404
Subject(s) - hydrogen peroxide , diabetic nephropathy , nephropathy , chemistry , immunology , medicine , biochemistry , diabetes mellitus , endocrinology
The production of hydrogen peroxide (H 2 O 2 ) by neutrophilic polymorphonuclear leukocytes (PMN) after stimulation with phorbol myristate acetate (PMA), n‐formyl‐I‐methionyl‐IIeucyl‐I‐phenylalanine (FMLP), aggregated human IgG, or Staphylococcus aureus was determined in 36 patients with non‐insulin dependent diabetes mellitus (NIDDM). H 2 O 2 production by PMN after stimulation was measured using flow cytometry. Thirty‐six patients with NIDDM were divided into four stages as follows: 1) stage I: non‐microalbuminuric stage;2) stage II: microalbuminuric stage; 3) stage III: proteinuric stage without impairment of renal function; and 4) stage IV: proteinuric stage with impairment of renal function. H 2 O 2 production after PMA stimulation in all stages of NIDDM patients was higher than that in healthy controls. This increase of H 2 O 2 production by PMN was particularly observed in stage IV of NIDDM patients after stimulation. Furthermore, H 2 O 2 production in patients in stage IV was higher than that in patients with non‐diabetic disease with impairment of renal function. It appears that reactive oxygen species produced by PMN after stimulation under some conditions may play an important role in the progression of diabetic nephropathy. © 1993 Wiley‐Liss, Inc.