Open AccessDetection of hepatitis C virus infection with recombinant immunoblot assay, synthetic immunoblot assay, and polymerase chain reaction
Author(s) -
Chaudhary R. K.,
Andonov A.,
Maclean C.
Publication year - 1993
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860070306
Subject(s) - virology , recombinant dna , polymerase chain reaction , hepatitis b virus dna polymerase , microbiology and biotechnology , biology , virus , hepatitis c virus , gene , genetics
The newly developed immunoblot assay, RIBA SIA (recombinant and synthetic polypeptide immunoblot assay), Chiron, Calif., was compared with the commercially available second generation recombinant immunoblot assay (RIBA‐2) for the detection of antibody to hepatitis C virus (anti‐HCV). The two immunoblot tests were also compared with the polymerase chain reaction (PCR) for the detection of HCV RNA. Ninety‐one percent of samples reactive by RIBA‐2 were positive for anti‐HCV by RIBA SIA. A total of 31% of RIBA‐2 indeterminate samples became reactive by RIBA SIA, 24% became non‐reactive, and 45% remained the same. Samples reactive by RIBA‐2 or SIA from different risk groups, were mostly positive (67‐100%) by PCR for HCV RNA. All indeterminate samples from hemophiliacs and intravenous drug users were PCR positive. RIBA SIA is more sensitive and specific than RIBA‐2 and correlates well with PCR results © 1993 Wiley‐Liss, Inc.