Open AccessStudy of the susceptibility of human colorectal tumor explants to lak‐cell lysis: Comparison of various cytotoxicity tests
Author(s) -
Ochalek Tomasz,
Kleist Sabine Von
Publication year - 1993
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860070305
Subject(s) - explant culture , cytotoxic t cell , lysis , cytotoxicity , spheroid , chemistry , immunology , lymphocyte , lymphokine activated killer cell , cell , cancer research , microbiology and biotechnology , biology , in vitro , biochemistry , interleukin 12
The susceptibility of tumor cells from cultures of 8 human colorectal tumor explants to a standard LAK preparation originating from a single healthy donor was assessed in 5 various cytotoxic tests. Target cells were suspended or attached (in three different 51 Cr‐release 4 hr tests), grown as a confluent monolayer (in a colorymetric 72 hr test) and grown as three‐dimensional spheroids (in a 51 Cr‐release 24 hr assay). The susceptibility to lysis observed for one tumor in the 5 tests varied from 4 to 15 times and was a tumor attribute independent of the susceptibility to lymphocyte attack. There was no correlation of results between the 4 hr tests and a 72 hr test performed on monolayer, or the spheroid test taking into account the resistance of the three‐dimensional structure to lymphocyte invasion. Our study excludes the possibility of quantitative interpolation of assessments of tumor cell susceptibility to lymphocytes received from various tests. It also refers to simple reductionist tests which may be compared, however, after ranking the qualitative categories. © 1993 Wiley‐Liss, Inc.