Open AccessQuick assay of serum HBs antibody levels using latex agglutination‐integrating sphere turbidimetric assay (ISTA)
Author(s) -
Yamashiki Masayoshi,
Kosaka Yoshitane,
Nishimura Akira,
Kondo Isao,
Tomita Akihito,
Kishioka Hiroshi
Publication year - 1993
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860070211
Subject(s) - antibody , hemagglutination , agglutination (biology) , latex fixation test , reproducibility , turbidimetry , chromatography , chemistry , immunology , microbiology and biotechnology , medicine , biology
A method for determining serum HBs antibody applying the principle of integrating sphere turbidimetric assay (ISTA) by latex agglutination was developed. The minimum detectable level of HBs antibody by this method is 12.5 IU/L, indicating that this method is 3 times or more sensitive with better reproducibility and specificity than the passive hemagglutination (PHA) method. With a cut‐off level of 25 IU/L, the possible highest simultaneous reading by this method was 1,000 IU/L Serum HBs antibody can be readily measured in 10 or so minutes by this method if a fully automated EL‐1000 analyzer is used. This rapid and simple method for determining serum HBs antibody will be useful not only clinically, but also in preventive medicine. © 1993 Wiley‐Liss, Inc.